CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Effect of the oral administration of the products derived from milk fermentation by kefir microflora on immune stimulation
Autor/es:
VINDEROLA, CELSO GABRIEL; PERDIGON, GABRIELA; DUARTE, JAIRO; FARNWORTH, EDWARD; MATAR, CHANTAL
Revista:
Journal of Dairy Research
Referencias:
Año: 2006 vol. 73 p. 475 - 479
ISSN:
0022-0299
Resumen:
Nutritional status has a major impact on the immune system. Probiotic effects ascribed to
fermented dairy products arise not only from whole microorganisms but also from metabolites
(peptides, exopolysaccharides) produced during the fermentation. We recently demonstrated the
immunomodulating capacity of kefir in a murine model. We now aimed at studying the
immunomodulating capacity in vivo of the products derived from milk fermentation by kefir
microflora (PMFKM) on the gut. BALB/c mice received the PMFKM for 2, 5 or 7 consecutive
days. IgA+ and IgG+ cells were determined on histological slices of the small and large
intestine. IL-4, IL-6, IL-10, IL-12, IFNc and TNFa were determined in the gut, intestinal fluid and
blood serum. IL-6 was also determined in the supernatant of a primary culture of small intestine
epithelial cells challenged with PMFKM. PMFKM up-regulated IL-6 secretion, necessary for
B-cell terminal differentiation to IgA secreting cells in the gut lamina propria. There was an
increase in the number of IgA+ cells in the small and large intestine. The increase in the
number of IgA+ cells was accompanied by an increase in the number of IL-4+, IL-10+ and
IL-6+ cells in the small intestine. Effects of PMFKM in the large intestine were less widely
apparent than the ones observed at the small intestine lamina propria. All cytokines that
increased in the small intestine lamina propria, also did so in blood serum, reflecting here the
immunostimulation achieved in the gut mucosa. We observed that the PMFKM induced a
mucosal response and it was able to up and down regulate it for protective immunity,
maintaining the intestinal homeostasis, enhancing the IgA production at both the small and
large intestine level. The opportunity exists then to manipulate the constituents of the lumen of
the intestine through dietary means, thereby enhancing the health status of the host.in vivo of the products derived from milk fermentation by kefir
microflora (PMFKM) on the gut. BALB/c mice received the PMFKM for 2, 5 or 7 consecutive
days. IgA+ and IgG+ cells were determined on histological slices of the small and large
intestine. IL-4, IL-6, IL-10, IL-12, IFNc and TNFa were determined in the gut, intestinal fluid and
blood serum. IL-6 was also determined in the supernatant of a primary culture of small intestine
epithelial cells challenged with PMFKM. PMFKM up-regulated IL-6 secretion, necessary for
B-cell terminal differentiation to IgA secreting cells in the gut lamina propria. There was an
increase in the number of IgA+ cells in the small and large intestine. The increase in the
number of IgA+ cells was accompanied by an increase in the number of IL-4+, IL-10+ and
IL-6+ cells in the small intestine. Effects of PMFKM in the large intestine were less widely
apparent than the ones observed at the small intestine lamina propria. All cytokines that
increased in the small intestine lamina propria, also did so in blood serum, reflecting here the
immunostimulation achieved in the gut mucosa. We observed that the PMFKM induced a
mucosal response and it was able to up and down regulate it for protective immunity,
maintaining the intestinal homeostasis, enhancing the IgA production at both the small and
large intestine level. The opportunity exists then to manipulate the constituents of the lumen of
the intestine through dietary means, thereby enhancing the health status of the host.c and TNFa were determined in the gut, intestinal fluid and
blood serum. IL-6 was also determined in the supernatant of a primary culture of small intestine
epithelial cells challenged with PMFKM. PMFKM up-regulated IL-6 secretion, necessary for
B-cell terminal differentiation to IgA secreting cells in the gut lamina propria. There was an
increase in the number of IgA+ cells in the small and large intestine. The increase in the
number of IgA+ cells was accompanied by an increase in the number of IL-4+, IL-10+ and
IL-6+ cells in the small intestine. Effects of PMFKM in the large intestine were less widely
apparent than the ones observed at the small intestine lamina propria. All cytokines that
increased in the small intestine lamina propria, also did so in blood serum, reflecting here the
immunostimulation achieved in the gut mucosa. We observed that the PMFKM induced a
mucosal response and it was able to up and down regulate it for protective immunity,
maintaining the intestinal homeostasis, enhancing the IgA production at both the small and
large intestine level. The opportunity exists then to manipulate the constituents of the lumen of
the intestine through dietary means, thereby enhancing the health status of the host.