CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Effects of kefir fractions on innate immunity
Autor/es:
VINDEROLA, CELSO GABRIEL; PERDIGON, GABRIELA; DUARTE, JAIRO; THANGAVEL, DEEPA; FARNWORTH, EDWARD; MATAR, CHANTAL
Revista:
IMMUNOBIOLOGY.
Referencias:
Año: 2006 vol. 211 p. 149 - 156
ISSN:
0171-2985
Resumen:
Innate immunity that protects against pathogens in the tissues and circulation is the first line of defense in the
immune reaction, where macrophages have a critical role in directing the fate of the infection. We recently
demonstrated that kefir modulates the immune response in mice, increasing the number of IgA+ cells in the intestinal
and bronchial mucosa and the phagocytic activity of peritoneal and pulmonary macrophages. The aim of this study
was to further characterize the immunomodulating capacity of the two fractions of kefir (F1: solids including bacteria
and F2: liquid supernatant), by studying the cytokines produced by cells from the innate immune system: peritoneal
macrophages and the adherent cells from Peyers patches. BALB/c mice were fed either kefir solid fraction (F1) or kefir
supernatant (F2) for 2, 5 or 7 consecutive days. The number of cytokine (IL-1a, IFNg, TNFa, IL-6 and IL-10)
producing cells was determined on peritoneal macrophages and adherent cells from Peyers patches. Both kefir
fractions (F1 and F2) induced similar cytokine profiles on peritoneal macrophages (only TNFa and IL-6 were upregulated).
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
producing cells was determined on peritoneal macrophages and adherent cells from Peyers patches. Both kefir
fractions (F1 and F2) induced similar cytokine profiles on peritoneal macrophages (only TNFa and IL-6 were upregulated).
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
a, IFNg, TNFa, IL-6 and IL-10)
producing cells was determined on peritoneal macrophages and adherent cells from Peyers patches. Both kefir
fractions (F1 and F2) induced similar cytokine profiles on peritoneal macrophages (only TNFa and IL-6 were upregulated).
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
a and IL-6 were upregulated).
All cytokines studied on adherent cells from Peyers patches were enhanced after F1 and F2 feeding, except
for IFNg after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.
g after F2 administration. Moreover, the percentage of IL-10+cells induced by fraction F2 on adherent cells
from Peyers patches was significantly higher than the one induced by fraction F1. Different components of kefir have
an in vivo role as oral biotherapeutic substances capable of stimulating immune cells of the innate immune system, to
down-regulate the Th2 immune phenotype or to promote cell-mediated immune responses against tumours and also
against intracellular pathogenic infections.