Effect of a fermented milk with a Lactobacillus helveticus R 389 proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice

RACHID, MIRTA; MATAR, CHANTAL; DUARTE, JAIRO; PERDIGON, GABRIELA

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY

Año: 2006 vol. 47 p. 242 - 253

0928-8244

Previous studies on a murine model have demonstrated that the administration of Lactobacillus helveticus and Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of b-glucuronidase enzyme in peritoneal macrophages, determination of g-interferon (INFg) and tumour necrosis factor-a (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofand Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of b-glucuronidase enzyme in peritoneal macrophages, determination of g-interferon (INFg) and tumour necrosis factor-a (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofL. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of b-glucuronidase enzyme in peritoneal macrophages, determination of g-interferon (INFg) and tumour necrosis factor-a (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofb-glucuronidase enzyme in peritoneal macrophages, determination of g-interferon (INFg) and tumour necrosis factor-a (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofg-interferon (INFg) and tumour necrosis factor-a (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofa (TNF-a) in blood serum, determination of CD41, CD81, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration of1, interleukin-6 (IL-6), IL-10, TNF-a and INFg by immunoperoxidase, and measurement of b-glucuronidase activity in intestinal fluid. The administration ofb-glucuronidase activity in intestinal fluid. The administration of L. helveticus delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.