Advanced application of porcine intestinal epithelial cells for the selection of immunobiotics modulating toll-like receptor 3-mediated inflammation

SHOICHI HOSOYA; JULIO VILLENA; ERIKO CHIBA; TOMOYUKI SHIMAZU; YOSHIHITO SUDA; HISASHI ASO; TADAO SAITO; HARUKI KITAZAWA

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION

SCIENTIFIC COMMUNICATIONS INT LTD

Lugar: Taiwan; Año: 2012

1684-1182

In this study, we characterized toll-like receptor (TLR)-3-mediated inflammatory immune responses in porcine intestinal epithelial (PIE) cells and in PIE-immune cell co-cultures. We demonstrated that these systems can be valuable tools for the in vitro study of the inflammatory response triggered by TLR3 in intestinal epithelial cells (IECs) and of the interaction between IECs and immune cells. In addition, we showed that PIE cells could be used for the selection of immunobiotic lactobacilli strains with anti-inflammatory activities. We found that Lactobacillus casei MEP221114 is an immunobiotic candidate for modulation of TLR3-mediated inflammatory responses. The present study also deepened our understanding of the mechanisms of immunobiotic action by demonstrating that the interaction between some lactobacilli strains and IECs can up-regulate the mRNA expression of TLR negative regulators and that this effect could help to regulate the production of inflammatory mediators during the generation of a TLR3-mediated immune response.