CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Lactobacillus reuteri CRL 1098 and Lactobacillus acidophilus CRL 1014 differently reduce in vitro immuno-toxic effect induced by Ochratoxin A
MECHOUD, MA; JUAREZ, GE; FONT DE VALDEZ, G; RODRIGUEZ, AV
FOOD AND CHEMICAL TOXICOLOGY
PERGAMON-ELSEVIER SCIENCE LTD
Lugar: Amsterdam; Año: 2012
Ochratoxin A (OTA) is a widespread mycotoxin contaminating several food products which causes detrimental health effects. The ability of L. reuteri CRL 1098 and L. acidophilus CRL 1014 to prevent OTA effects on TNF-alpha and IL-10 production and apoptosis induction in human peripheral blood mononuclear cells (PBMC) was investigated. Membrane rafts participation in these responses was also evaluated. L. reuteri reduced by 29% the OTA inhibition of TNF-alpha production whereas L. acidophilus increased 8 times the TNF-alpha production by OTA treated-PBMC. Also, both bacteria reversed apoptosis induced by OTA by 32%. However, neither of the bacteria reversed the OTA inhibition on IL-10 production. On the other hand, the lactobacilli were less effective to reverse OTA effects on disrupted-rafts PBMC. This study shows that two lactobacilli strains can reduce some negative OTA effects, being membrane rafts integrity necessary to obtain better results. Also, the results highlight the potential capacity of some lactobacilli strains usually included in natural dietary components in milk-derived products and cereals feed, to reduce OTA toxicity once ingested by humans or animals.