CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Conditioned media from Lactobacillus reuteri CRL 1098 decreases TNF-alpha production and apoptosis in peripheral blood mononuclear cells: involvement of lipid rafts
MECHOUD, MA; MATEOS, MV; FONT DE VALDEZ, G; VILLENA, JC; SALVADOR, GA; RODRIGUEZ, AV
ELSEVIER SCIENCE BV
Lugar: Amsterdam; Año: 2012 vol. 14 p. 446 - 446
The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate cytokines production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced TNF-alpha production while no effect was observed in IL-10. In addition, Lr-S treatment activated ERK and p38 MAPK pathways and reduced apoptosis in PBMC. Lipid rafts participation in the reduced production of TNF-alpha by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-beta-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-alpha production suggesting that activation and movilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells.