CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
Autor/es:
VANNINI V; RODRIGUEZ A; VERA JL; FONT DE VALDES G; TARANTO MP; SESMA F
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Dordrecht; Año: 2011 p. 1 - 1
ISSN:
0141-5492
Resumen:
Some strains of Lactobacillus (Lb.) reuteri are able to produce cobalamin (vitamin B12). Cobalamin 32 biosynthesis relies on the sequential action of more than 25 enzymes, in a very complex metabolic pathway. In this 33 work we present the cloning, expression and preliminary characterization of the gene that codes for the S-Adenosyl34 methionine  Uroprophyrinogen III methyltransferase/synthase (CobA/HemD) in Lb. reuteri CRL1098, a key 35 bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols in some microorganisms