CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
JULIO VILLENA; RIE SUZUKI; HITOMI FUJIE; ERIKO CHIBA; TAKUYA TAKAHASHI; YOHSUKE TOMOSADA; TOMOYUKI SHIMAZU; HISASHI ASO; SHUICHI OHWADA; YOSHIHITO SUDA; SHUJI IKEGAMI; HIROYUKI ITOH; SUSANA ALVAREZ; TADAO SAITO; HARUKI KITAZAWA
CLINICAL AND VACCINE IMMUNOLOGY
AMER SOC MICROBIOLOGY
Lugar: Washington; Año: 2012
Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyers patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.