CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Application of fluorescent techniques to evaluate the survival of probiotic lactobacilli to bile acid
BUSTOS, A.Y. ; RAYA, R.; BRU, E.; FONT DE VALDEZ, G; TARANTO, M.P.
Lugar: Berlin; Año: 2011 vol. 33 p. 1389 - 1389
The use of lactic acid bacteria (LAB) in the functional food design depends of their ability to survive in the gastrointestinal tract where bile is an important natural barrier. Bile is mainly constituted by conjugated bile acids, which can be hydrolyzed to free bile acids and taurine or glycine.In this work, changes in the transmembrane electrical potential (ΔΨ) of probiotic LAB strains due to the effect of conjugated and free bile acids were measured. The results showed that the majority of the tested LAB strains showed greater sensibility to free bile acids than to their respective conjugated acids. Variations in the ΔΨ of the microorganism correlated well with bacterial viability determined by standard plate count method.According the results obtained, we propose the DiSC3-based fluorescent technique as a rapid and effective method to evaluate the resistance of probiotic lactobacilli to bile as first step for strain selection to be included in functional foods