CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
IN VITRO EVALUATION OF BENEFICIAL PROPERTIES IN SPORE FORMER BACTERIA FOR THEIR POTENTIAL INCLUSION IN PROBIOTIC PRODUCTS AS ADDITIVES FOR CALVES
MALDONADO NC ; SILVA DE RUIZ C; NADER MACÍAS ME
INDIAN JOURNAL OF MICROBIOLOGY
Lugar: Dordrecht; Año: 2011
Spore-former bacteria, mainly the Bacillus species are being used as probiotic additives in animal nutrition. In this work, some Bacillus strains were isolated from calves faeces and evaluated for their beneficial properties. Two of them were selected, identified as Bacillus subtilis to further evaluate their inclusion in the design of a novel probiotic product for newborn calves. Surface properties, biosurfactant and emulsification production were assayed. Spores resulted to be more hydrophobic than vegetative cells. One of the selected strains had shown biosurfactant and emulsifying properties. Inhibitory assays against pathogenic bacteria and peroxide production were evaluated as antimicrobials compounds, and resulted inhibitory against gram-positive microorganisms. The antibiotic susceptibility indicated that the two strains were sensitive to the antibiotics assayed, except Bacillus M12 that was resistant to Kanamycin. Bacillus subtilis M14 and B. subtilis M12 were selected to evaluate their inclusion in the design of a new probiotic product to use as adjunct.