CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
ERIKO CHIBA; JULIO VILLENA; SYOICHI HOSOYA; NAOYA TAKANASHI; TOMOYUKI SHIMAZU; HISASHI ASO; MASANORI TOHNO; YOSHIHITO SUDA; YASUSHI KAWAI; TADAO SAITO; KENJI MIYAZAWA; FANG HE; HARUKI KITAZAWA
RESEARCH IN VETERINARY SCIENCE
ELSEVIER SCI LTD
We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the pri- mary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluores- cent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real- time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially mod- ulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valu- able bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts.