Rapid identification of new isolates of Acidipropionibacterium acidipropionici by fluorescent in situ hybridization (FISH)

APELLA M.C.; ARGAÑARAZ MARTINEZ E.; BABOT J.D.; PEREZ CHAIA A.

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2022 vol. 54 p. 263 - 267

0325-7541

Acidipropionibacterium acidipropionici is widely used for many applications, such as propionic acid production, cereal silage, and also as probiotic. Due to this plethora of applications, new isolates of A. acidipropionici with better features are being searched for. These new isolates must be accurately identified, but most approaches result expensive and time-consuming when the number of isolates is elevated. On the contrary, the fluorescent in situ hybridization allows the low-cost, reliable, and rapid identification of microorganisms in pure cultures and environmental and medical samples. Therefore, the aim of this work was the application of a fluorescent in situ hybridization probe for the reliable identification of new A. acidipropionici isolates. To this end, the probe Pap446, specific for A. acidipropionici was validated by hybridization assays with strains of this species from different origin, other species of the same genera or family, and not related genera. Eight isolates with characteristics of propionibacteria were obtained from the milk and feces of cows. The probe Pap446, hybridized only with isolates III and VI, and the identity of these isolates was further confirmed by PCR with propionibacteria group- and species-specific primers and 16S rDNA sequencing.