CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Influence of probiotic vaginal lactobacilli on in vitro adhesion of urogenital pathogens to vaginal epithelial cells
GABRIELA ZÁRATE,; MARIA ELENA NADER-MACIAS
LETTERS IN APPLIED MICROBIOLOGY
Lugar: Bedford; Año: 2006 vol. 2 p. 174 - 174
Aims: Lactobacilli, the predominant microorganisms of the vaginal microbiota, play a major role in the maintenance of a healthy urogenital tract by preventing the colonization of pathogenic bacteria. The aim of the present study was to assess the ability of four vaginal Lactobacillus strains, previously selected for their probiotic features, to block in vitro the adherence of three human urogenital pathogens to vaginal epithelial cells (VEC). Methods and Results: Three types of assays were performed in order to determine the inhibitory effect of lactobacilli on adhesion of urogenital pathogens to VEC: blockage by exclusion (lactobacilli and VEC followed by pathogens), competition (lactobacilli, VEC and pathogens together) and displacement (pathogens and VEC followed by the addition of lactobacilli). Bacterial adhesion to VEC was quantified by microscopy (x1000) after Gram´s stain. All the strains were able to inhibit by exclusion and competition the adhesion of Staphylococcus aureus to VEC but no one was able to decrease the attachment of Escherichia coli by neither of the mechanisms assayed. Only L. acidophillus CRL 1259 and L. paracasei CRL 1289 inhibited the attachment of Group B streptococci to VEC by exclusion and competition and competition respectively. Conclusions: Lactobacillus of vaginal origin were able to inhibit the attachment of genitouropathogenic S. aureus and GBS to the vaginal epithelium. Significance and Impact of the Study: The results support the probiotic potential of these Lactobacillus strains as anti-infective agents in the vagina and encourage further studies about their capacity to prevent and manage urogenital tract infections in females.