PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biofertilizer production bioprocess using vinasse and cheese whey in the media composition.
Autor/es:
EMILCE VIRUEL; FAUSTINO SIÑERIZ; MARIA ESTER LUCCA
Lugar:
San Diego, CALIFORNIA, USA
Reunión:
Congreso; 8th bi-annual Recent Advances in Fermentation Technology conference, co-sponsored by the Society for Industrial Microbiology (SIM) and the American Chemical Society, Division of Biochemical Technology (ACS BIOT); 2009
Institución organizadora:
Society for Industrial Microbiology (SIM) and the American Chemical Society, Division of Biochemical Technology (ACS BIOT)
Resumen:
Biofertilizer
production bioprocess using vinasse and cheese whey in the media composition.
Viruel E.1, Lucca M. E.1,2,Siñeriz F.1,21PROIMI-CONICET
(Pilot Plant of Industrial Microbial Processes), Belgrano and Caseros (4000) Tucumán. 2 Superior
Microbiology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Argentine
eviruel@proimi.org.ar mlucca@proimi.org.ar fsineriz@arnet.com.ar
The plant-growth
promoting rhizobacteria (PGPR) can solubilize unavailable phosphate and produce
phytohormones, antibiotic and siderophores. A bacterial strain, Pseudomonas tolaasii IEXb, (Access number FM202487) isolated from high altitude soils (Puna Argentine North West) with P-solubilizing
activity and siderophores presence
was selected. The growth-promoter effect was also previously demonstrated on
maize plants. The use of inexpensive sources (industrial and
agricultural wastes) for Pseudomonas
tolaasii IEXb production was studied in this work. Molasses
wastewater (vinasse) and cheese whey protein concentrate were used as natural media. The strain was inoculated (102
cfu /ml) in 100ml Erlenmeyer flasks with 50ml of vinasse media (M1
and M2), whey plus cerelose medium (MEL) and vinasse plus whey media
(MEL10 and MEL 50). Biomass concentrations in the order
of 1010 cfu/ml were reached in all media tested. The strain was able
to growth in vinasse concentrations up to 50% v,v. Batch culture in 5L M1 medium (10% vinasse v,v; MgSO4 0.1g.L-1;
KH2PO4 1 g.L-1) was selected to scale up the production in 10L fermentor (Microferm) under
controlled culture conditions (pH 7, 60% oxygen saturation, 200 rpm, 37ºC). After inoculation, the
initial cfu/ml in the production medium was 1x102 /ml. There were
obtained 4 x 1013 total viable colonies. Chemical oxygen demand
(COD) of M1 medium showed a decrease of 25% respect of the initial media COD. Results
showed the feasibility of scaling up the biomass production bioprocess to a Pilot
Plant fermentor. Considering future trends of biofertilizer application for
sustainable crop production, low cost of natural media involved is a powerful
tool to be considered.
Supported by CIUNT 26/D434