PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Scleroglucan production by Sclerotium rolfsii ATCC 201126 from amylaceous and sugarcane molasses-based media
Autor/es:
JOCHEN SCHMID; BABOT JD; FARIÑA JI; VALDEZ AL; DELGADO OD
Lugar:
Straubing
Reunión:
Simposio; X European Symposium on BioPolymers 2019; 2019
Institución organizadora:
Wissenschafstzentrum Straubing TUM & CARMEN
Resumen:
Biotechnology tries solving the growing demand on biodegradable polymers of low costs, high quality and viscosifying properties. The large-scale production of microbial exopolysaccharides (EPSs) by controlled fermentation is a promising example of biobased polymers, with world markets representing several hundred million dollars annually [1,2]. Scleroglucan (SC) is one of these microbial EPS, produced mainly by fungi of the genus Sclerotium, under optimized and standardized cultivation conditions [2-3]. Its excellent properties make it especially attractive for various industrial applications ranging from assisted oil recovery to the cosmetic industry [2-3]. To further optimize it´s production and economy, in this work, nine alternative low-cost C- and twelve N-sources were comparatively assessed. When comparing conventional sucrose-based Production Medium PM (8.41 g C/L + NaNO3 as N-source) at shake-flask-scale vs. alternative C-source versions, soluble starch and sugarcane molasses led to efficient SC production. On the other hand, oxalic acid byproduct varied with C- and N-sources, with no clear detrimental relationship with SC production. Afterwards, at 3 L bioreactor scale, a starch-based medium led to the highest SC production (7.95 g/L), best recovery efficiency (~ 52%) and high values of volumetric and specific productivities (0.11 g SC/L h and 0.018 g SC/g h respectively). Sugarcane molasses, though leading to lower SC production (5.11 g/L), could be envisaged as a promising agroindustrial sub-product as alternative substrates for efficient, low-cost, and scalable SC production, thus opening new perspectives for medium reformulation and sustainable and economic SC production.