INFLUENCIA DEL CLORURO DE CALCIO EN LA POLARIDAD CELULAR DE Aspergillus niger MYA 135

COLIN VERONICA; BAIGORÍ M; PERA L

Tafí del Valle-Tucumán, Argentina

Congreso; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; 2008

Asociación de Biología de Tucumán

Introduction: The hyphal is a tubular cell whose form is conserved thanks to the cell wall, site of diverse enzymatic activities. The â-N-acetyl-D-glucosaminidase (NAGase), litic enzyme of wall, it can be used as a biomarker of the hyphal morphology and the stimulating effect of calcium on this activity was reflected in an increase of the degree of ramification of mycelium and present of abundant bulbous cells. Thus, the loss of cellular polarity could be the result of a wall strongly weakening. Objetive: To determine if the cellular polarity is recovered with supplement of an osmotic stabilizer to culture medium. Materials and Methods: The mycelium that develops in salt medium at 30° C is considered standard. The morphologic changes were studied in mycelium obtained in the presence of CaCl2 with and without theI supplement of NaCl, as osmotic stabilizer. Results and conclusion: In the mycelium developed in the presence of NaCl, bulbous cells were not observed and cellular morphology was recovered. This work was development by grant PICTO-UNT 761 y PIP 6062.