PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Synergistic effect of xylanases produced in co-culture of Bacillus sp. AR03 and Paenibacillus sp. AR247
Autor/es:
HERO JS; PISA JH; PEROTTI NI; ROMERO CM; MARTÍNEZ MA
Reunión:
Congreso; SAMIGE; 2015
Resumen:
Synergistic effect of xylanases produced in co-culture of Bacillus sp. AR03 and Paenibacillus sp. AR247Johan S. Hero1, José H. Pisa1, Nora I. Perotti1,2, Cintia M. Romero1,3, María A. Martínez1,21Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Tucumán2Facultad de Ciencias Exactas y Tecnología, Tucumán3Facultad de Bioquímica, Química, Farmacia y Biotecnología, TucumánE-mail address: ale.martinez.unt@gmail.comIn nature, the plant biomass is degraded by a process that requires the cooperative action of multiple microorganisms capable of producing a variety of enzymes to attack the complex structure of lignocelluloses. This work assessed the production and the enzymatic activity over the main hemicellulolytic fraction of plant biomass, xylan, in monoculture and co-culture systems of bacteria isolated from regional niches associated with sugar cane bagasse.The enzyme activity was measured by means of the reducing sugars released using the dinitrosalicylic acid method. All cultivation assays were performed at 200 rpm and 30 °C in a diluted peptone broth supplemented with 1% CMC (w/v). The viability and the growth of both isolates during cultivation assays were estimated by the number of colony forming units evaluated each 24 h, fact that was possible since both isolates exhibited different colony morphology.The specific xylanolytic activity of the co-culture of Bacillus sp. AR03 and Paenibacillus sp. AR247 was of 7.03  0.46 IU/mg and 8.36  0.49 IU/mg at 48 h and 96 h of cultivation, respectively. In contrast, each isolate assayed simultaneously under identical conditions, produced significantly lower xylanase activities, even when both isolates grew similarly in both, individual and co-cultures, reaching approximately 1012 CFU/ml in all cases. These values were of 4.18  0.24 IU/mg and 4.55  0.29 IU/mg of xylanolytic activity at 48 h and 96 h, respectively, for Bacillus sp. AR03, while Paenibacillus sp. AR247 reached values of 0.59  0.09 IU/mg and 0.40  0.03 IU/mg at the same periods of cultivation. When mixtures (1:1) of the cell-free supernatant of individual cultures were assayed, it was observed that the enzymatic activity reached a maximum of 4.16  0.39 IU/mg after 48 h of cultivation. This value was close to that obtained by the sum of the enzymatic activity of individual cultures, which was 4.77 IU/mg, for the same cultivation time.The results obtained are consistent with the observation of a synergistic effect on the degradation of xylan in the co-culture evaluated, with an estimated degree of synergism of 1.69 at 96 h. This synergy, which has been described for enzyme mixtures on industrial substrates, was observed here during the co-cultivation of Bacillus sp. AR03 and Paenibacillus sp. AR247. This system displayed a higher xylanolytic activity with respect to the individual cultivation of each isolate and a different zymographic pattern along the cultivation period. The results of the xylanolytic activity obtained for individual strains and the co-culture might indicate that the effect observed might not only rely on an addition of enzyme activities; they also suggest a synergistic cooperation during the growth in the co-cultivation of the microorganism evaluated.AcknowledgmentsThis research was supported by Argentinean grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and the Consejo de Investigaciones de la Universidad Nacional de Tucumán (CIUNT).