Physiological and biochemical characterization of pseudoalteromonas sp. p8 a cold-active xylanases producer

ADRIANA E. ALVARENGA; CRISTÓBAL H. A.; ABATE C. M.

Rosario

Congreso; V Congreso Argentino de Microbiología General; 2008

SAMIGE

Xylanolitic enzymes are involved in a variety of industrial reactions. Several studies have reported that enzymes produced by psychrophiles organisms have high specific activity at low and moderate temperature, and an elevate thermosensibility. Those properties are very useful to perform biological transformations at low temperatures. The aims of this work were: 1) Qualitative and quantitative determinations of xylanolitic activity from the Pseudoalteromonas sp. P8 strain. 2) Biochemical and physiological assays to characterize the microorganism. P8 strain was grown in minimum medium added with xylan and hemicellulose. Enzymatic activities were detected at 4 and 20 ºC by the appearance of clarification halos after Congo red staining. Xylanase activity was determined at several temperatures (15, 25, 30 and 37 ºC) by the 3,5 dinitrosalicylic acid (DNS) method. The highest specific activity was registered at 25 ºC. Biochemical and physiological characterization were carried out using the API 20 NE (BioMerieux) test, and by growth curves at 4 and 20ºC. The obtained results demonstrate that Pseudoalteromonas sp. P8 is a promissory strain for cold- active xylanase expression.Pseudoalteromonas sp. P8 strain. 2) Biochemical and physiological assays to characterize the microorganism. P8 strain was grown in minimum medium added with xylan and hemicellulose. Enzymatic activities were detected at 4 and 20 ºC by the appearance of clarification halos after Congo red staining. Xylanase activity was determined at several temperatures (15, 25, 30 and 37 ºC) by the 3,5 dinitrosalicylic acid (DNS) method. The highest specific activity was registered at 25 ºC. Biochemical and physiological characterization were carried out using the API 20 NE (BioMerieux) test, and by growth curves at 4 and 20ºC. The obtained results demonstrate that Pseudoalteromonas sp. P8 is a promissory strain for cold- active xylanase expression.Pseudoalteromonas sp. P8 is a promissory strain for cold- active xylanase expression.