Colonization and Domestication of Seven Species of Larval-Prepupal and Pupal Native New World Hymenopterous Fruit Fly (Diptera: Tephritidae) Parasitoids

MARTIN ALUJA,; JOHN SIVINSKI,; SERGIO OVRUSKI; LARISSA GUILLEN; MAURILIO LOPEZ; JORGE CANCINO,; ARMANDO TORRES-ANAYA; GUADALUPE GALLEGOS-CHAN; LIA RUIZ; GUADALUPE CORDOVA; ALEJANDRO MARTINEZ-RAMIREZ

Mazatlán, Sinaloa, México

Workshop; 7ma Reunión del Grupo de Trabajo en Moscas de la Fruta del Hemisferio Occidental; 2008

Programa Moscamed-Moscafrut, SAGARPA-SENASICA, México - IAEA

Techniques used to colonize and domesticate seven native New World species of hymenopterous parasitoids that attack flies within the genus Anastrepha (Diptera: Tephritidae) were developed: Doryctobracon areolatus (Szépligeti), Doryctobracon crawfordi (Viereck), Opius hirtus (Fischer), Utetes anastrephae (Viereck) (all Braconidae, Opiinae), Aganaspis pelleranoi (Bréthes) and Odontosema anastrephae Borgmeier (both Figitidae, Eucoilinae) (all larval-pupal parasitoids), and the pupal parasitoid Coptera haywardi (Ogloblin) (Diapriidae, Diapriinae) were reared successfully on Mexican fruit fly, Anastrepha ludens (Loew) larvae or pupae reared on artificial diet. Descriptions of the different rearing techniques used throughout the domestication process are described to help researchers to colonize local parasitoids.  We also describe handling procedures such as number of hosts in parasitization units and compare optimal host and female age, differences in parasitism rate, developmental time, life expectancy and variation in sex ratios in each parasitoid species over various generations. In the case of D. crawfordi and C. haywardi we also provide partial information on mass-rearing techniques such as cage type, parasitization unit, larval irradiation dose and adult handling.