PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE FIGHT AGAINST PATHOGENS IS NOT OVER: ARGENTINE ANTARCTIC EXPEDITIONS DISCLOSE A NOVEL SOURCE FOR COLD-ACTIVE ANTIMICROBIALBIOPROSPECTING
Autor/es:
DANILOVICH, MARIANA; SANCHEZ, LEANDRO; ACOSTA, FEDERICO; DELGADO, OSVALDO
Reunión:
Congreso; X CONGRESO ARGENTINO DE MICROBIOLOGIA GENERAL; 2014
Resumen:
An organism that lives in an extreme environment is an extremophile. Within extremophiles we can find organisms belonging to archaea, prokaryotes and eukaryotes. Key factors, such as frequent and inappropriate use of antibiotic causing increase in bacterial resistance to those commonly used, as well as an irregular emergence generated from a detriment of pharmaceutical companies involved in drugs discovery, makes the development of new antibiotics is a growing worldwide problem and a constant challenge. The need to reduce additives in food industry has triggered the pursuit of natural antimicrobial substances produced by microorganisms from different sources with the aim of preserving processed foods by inhibiting microorganisms associated with food-borne illness. Most antibiotics emerged from screening programs of natural sources, including the isolation of new microorganisms, modification of well-known producers or metabolic engineering of fermentation processes. Extremophiles are considered an important source of metabolites, enzymes and bio-products since they are adapted to their unusual living conditions. This work focused on the isolation of psychrotolerant and psychrophilic microorganisms producing cold-active substances with the ability to inhibit the growth of common food-borne pathogens and phytopatogens for potential use in food preservation, pharmaceutical industry or as agriceutical. Twenty-seven antimicrobial producers were isolated by using microbiological selection techniques from sea water and sediment samples obtained during the 2011 and 2014 summer Antarctic campaigns of the oceanographic survey ship ARA Puerto Deseado. Isolates were grouped in 11 OTUs by ITS-ARDRA techniques; four of them were selected for identification based on rDNA regions sequence analysis together with the biochemical characterization. Isolates 2D, 5D and 6D were closely related to Halomonas titanicae (99.8, 98.9, 96.7% respectively), whilst isolate 18SH was related to Candida sake (99%). Antimicrobials produced by isolates 2D, 5D and 6D exhibited low molecular weight (