PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
APPLICATION OF COMPLETE FACTORIAL DESIGN FOR OPTIMIZATION OF CULTURE MEDIUM FOR Cr(VI) REMOVAL BY INDIGENOUS YEASTS
Autor/es:
CRUZ, ELÍAS L.; FERNANDEZ, PABLO M; FIGUEROA, LUCÍA I. C.
Lugar:
Tucumán
Reunión:
Jornada; XXXI Jornadas científicas; 2014
Institución organizadora:
Asociación de Biología DE TUCUMAN
Resumen:
Chromium pollution is increasing incessantly due to continuing industrialization. Of various oxidation states, Cr(VI) is very toxic due to its carcinogenic and mutagenic nature. Microbial reduction of toxic chromium offers a potential cost-effective bioremediation approach. In order to reduce operating costs in a large scale process, composition and concentration of the components of a culture medium was studied. Complete factorial design was employed to optimize Cr(VI) removal (1 mM initial concentration) at 24 h by indigenous yeasts Cyberlindnera jadinii M9 and Wickerhamomyces anomalus M10. The interaction between four variables (SO4(NH4)2; CaCl2; NaCl, and innoculum) for M9, and three variables (sucrose, KHPO4, and innoculum)for M10 were studied and modelled. In both strains, total removal of Cr(VI) was achived at 24 h. Also, removal time optimization at 12 h was optimized. The optimum combination predicted was confirmed through experiment, whereby maximum removal of Cr(VI) reached at 12h was 73.91% and 87.32% for M9 and M10, respectively. In the case of M9, NaCl could be excluded from the medium formula because no present any effect on Cr(VI) removal. The final composition of optimized medium for M9 is (g/L): sucrose, 60; KHPO4, 1; yeast extract, 1; Mg(SO4)2, 0,5;SO4(NH4)2, 1,05; CaCl2 0,12; innoculum, 20% and for M10 (g/L): sucrose, 90; KHPO4, 1,2; SO4(NH4)2, 1,2; yeast extract, 1; Mg(SO4)2, 0,5; CaCl2, 0,1; NaCl, 0,1; innoculum, 20%. These results strongly suggest that Cr(VI) removal could be reached in a short time indicating that this economic medium formulated could be used in developing a bioremediation process at large scale