PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Catalytic properties of constitutive and olive oil-induced extracellular and mycelium-bound lipases from Penicillium corylophilum: a comparative study
Autor/es:
CINTIA M. ROMERO, LICIA M. PERA, ALESSANDRA M. BARON, NADIA KRIEGER , MARIO D. BAIGORI
Lugar:
Trivandrum
Reunión:
Congreso; New Horizons of Biotechnology (NHBT)-2011; 2011
Resumen:
Screening of lipolytic strains using Rhodamine-B/olive oil plate technique allowed the selection of Penicillium corylophilum. Lipase production in a submerged culture containing 2% olive oil increased 2.6 and 1.4 fold in the case of extracellular and mycelium-bound lipase, respectively, compared with constitutive conditions. In general, the four biocatalyst systems showed high residual activity at acid and neutral pH. Preincubation at 40°C caused an increase in the extracellular olive oil-induced lipolytic activity. On the contrary, residual activity of extracellular constitutive lipase decreased within the 30–55°C range. Constitutive mycelium-bound lipase showed to be stable at high temperatures like 50 and 55°C, while the activity of olive oil-induced  mycelium-bound lipase decreased at 55°C. Constitutive and olive oil-induced extracellular lipase showed highest stability in water miscible solvents. Constitutive and olive oil-induced mycelium-bound lipase showed residual activity in both water-miscible and immiscible solvents. Different catalytic properties of several biocatalysts from the same microorganism were obtained by changing the surrounding medium. Selectively modified biocatalysts could used for different industrial process cheapen the costs of the biocatalysis.