PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
OPTIMIZED LOVASTATIN PRODUCTION BY SOLID-STATE FERMENTATION WITH ASPERGILLUS TERREUS WILDTYPE STRAIN”.
Autor/es:
CABRAL M. EUGENIA; JOYA M. CONSTANZA; CASTELLANOS DE FIGUEROA, L I; FARIÑA J I
Reunión:
Congreso; REUNIÓN ANUAL SAIB 2010; 2010
Resumen:
Statins can inhibit the de novo synthesis of cholesterol by competitive inhibition of HMG-CoA reductase, which catalyzes the rate limiting step of cholesterol biosynthesis. For this reason, statins are widely used, between other clinical applications, as effective drugs in the hypercholesterolemia treatment. Objective.This work was aimed to optimize and simplify composition medium and fermentation conditions at shake flasks scale to produce, subsequently, lovastatin at high concentrations by solid state fermentation (FSS). Methodology. Aspergillus terreus MEC, was cultured by submerged fermentation (SF) in synthetic medium, lactose-yeast extract, in shake flasks at 250 rpm and 25ºC during 14 days. The optimization included different C and N sources test and the decrease of salts and trace elements concentration. Additionally, it was evaluated the influence of soy flour addition to the media formulation and the replace of lactose for milk whey powder. For FSS different solid support were assayed. Lovastatin was detected, previous extraction,  by using RP-HPLC with Diode Array Detector. Results and conclusions. A lovastatin production of 63 mg/L was reached in optimized liquid medium. Production was significantly increased to ~1000 mg/L by using SSF systems consisting in liquid medium prepared with whey milk adsorbed on textured soy flour, cheap and readily available substrates.