OPTIMIZATION OF CULTURE MEDIUM OF Bionectria sp. FOR PRODUCTION OF FIBRINOLYTIC ENZYMES.

ARNAU, VG; ROVATI, JI; FIGUEROA, LIC; FARIÑA, JI

CHUBUT

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Cardiovascular diseases are one of the leading causes of death worldwide. There have been many attempts at laboratory level to increase the production of fibrinolytic enzymes and reduce total cost of the process, which include the selection of an ideal culture medium, optimization of environmental conditions, and overexpression using genetically modified strains. The aim of this work was the optimization of culture medium and environmental conditions for fibrinolytic enzyme production by Bionectria sp. isolated from the ecosystem of Las Yungas. From a culture medium previously reported to fibrinolytic enzymes production, optimization was carried out at Erlenmeyer level (100 mL working volume) via factorial design methodology. All trials used a base of common salts (% w / v: NaCl 0.2, KH2PO4 0.05; MgSO4·7H2O 0.05). In this work, four factorial designs were developed to analyze the influence of different nitrogen and carbon sources, at different concentrations, as well as the effect of incubation temperature on production of fibrinolytic activity. The results obtained in this study showed that the culture medium for the production of fibrinolytic enzymes with Bionectria sp. could be successfully optimized by factorial design methodology, achieving a reduction in the cost of the production process due to a decrease of medium and operational costs and enlarged productivity of the process.