TRANSCRIPTIONAL AND PROTEOMIC ANALYSES OF REDOX ENVIOREMENT IN BETA-THALASSEMIA TRAIT.

MÓNACO ME; LAZARTE SS; AGUERO AGUILERA AC; TERÁN MM; HARO C; ALVAREZ ASENSIO N; SINELI PE; LEDESMA ACHEM ME; ISSÉ B

Buenos Aires

Congreso; LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2021

β-Thalassemia trait (BTT) is a heterogeneous group of genetic defects leading to decreased β -globin production, ineffective erythropoiesis and oxidative stress. Reactive oxygen speciesproduction and oxidative environment have an impact on all blood cell lineages. The aim was toevaluate, at transcriptional and proteomic levels, the pro-oxidative and anti-oxidative status in BTTpatients. A descriptive study was performed with 66 subjects (40 apparently healthy and 26 withBTT). Real-time PCR was used for gene expression analyses of transcription factors forkheadhomeobox typeO (FOXO3a) and nuclear factor erythroid2-related factor-2 (NRF2); antioxidantenzymes: catalase (CAT), peroxiredoxin-2 (PRX-2), superoxide dismutase (SOD); and cytokines TNF-α, IL-6. Quantitative mass spectrometry was performed on cytosol erythrocyte membranesdepleted of hemoglobin. Bioinformatic analysis was performed with Perseus, BlastKoala andProteome Discoverer V1.4 programs.Relative expression of NRF2 was 4.7-fold higher in BTT than in control group, whereas FOXO3aexpression was similar in both. Transcriptional expression of SOD, PRDX2 and proinflammatorycytokines were significantly upregulated in BTT compared to controls (p