A124 POTENTIAL ANTIFUNGICAL EFFECT OF KILLER YEAST ON THE HUMAN PATOGENIC FUNGI Candida AND Cryptococcus

SANGORRIN MARCELA; MARTIN DIAZ ALFARO; ALVAREZ CHRISTIAN; LUCCA MARIA ESTER

BIOCELL

Burgos M. and Piezzi R

Lugar: BUENOS AIRES; Año: 2020 vol. 43 p. 26 - 26

0327-9545

Potential antifungal effect of killer yeast on the human pathogenic fungi Candida and Cryptococcus BIOCELL 43 (suppl. 1), 2019 ABSTRACTS ISSN 0327- 9545 A1 ? A133 ISSN 1667-5746 (online version) XXXV ANNUAL SCIENTIFIC MEETINGA124 POTENTIAL ANTIFUNGICAL EFFECT OF KILLER YEAST ON THE HUMAN PATOGENIC FUNGI Candida AND Cryptococcus Alvarez C2 ; Diaz Alfaro M1 , Sangorrín M3 , Lucca ME1,4 1PROIMI-CONICET; 2Micología, Instituto de Microbiología, UNT; 3PROBIEN-CONICET-UNCOMA; 4Microbiología Superior, Instituto de Microbiología, UNT. Ayacucho 491, Tucumán. E-mail: bqcochal@gmail.com Candida and Cryptococcus are cosmopolitan opportunistic yeasts that grow at 37ºC and cause mycosis in humans. Currently available drugs are scarce and not very selective. The objective of this study was to evaluate the antifungal activity of 3 killer yeasts: Wickerhamomyces anomalous strain 1026, Metschnikowia pulcherrima strain 1010 and Vishiniacozyma victoriae strain 1263 against 8 clinical isolations: Candida albicans (2), Candida parapsilopsis (2), Candida tropicalis (2) and Cryptococcus neoformans (2). The assays were performed using the YPD-MB agar growth inhibition technique. 1X106 cell/ml suspensions of each pathogenic microorganism were inoculated. Then, three perforations were made in the agar and 20 µL of each killer yeast was inoculated. Incubation was conducted at 28°C for 48-72 h. The presence of halo inhibition of pathogen growth was considered as a positive sensitivity to killer toxins. The three yeasts studied showed inhibition of C. albicans, C. parapsilopsis, C. tropicalis and C. neoformans. These results demonstrate the relevance of studying the nature of the compound produced by killer yeasts with inhibitory effect on the growth of opportunistic pathogens and the need to continue with trials in order to optimize the production process in fermenter to achieve high yields and productivity in order to demonstrate their technical feasibility and commercial viability.