Comparison of seven structurally related coumarins on the inhibition of Quorum sensing of Pseudomonas aeruginosa and Chromobacterium violaceum

NIETO PEÑALVER, C.; D'ALMEIDA, R.E.; ARENA, M.E.; BARDÓN, A.; LUCIARDI, M.C.; VIOLA, C.M.; MOLINA, R.D.I.; NIETO PEÑALVER, C.; D'ALMEIDA, R.E.; BARDÓN, A.; VIOLA, C.M.; ARENA, M.E.; LUCIARDI, M.C.; MOLINA, R.D.I.

BIOORGANIC CHEMISTRY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017 vol. 73 p. 37 - 42

0045-2068

Comparison of seven structurally related coumarins on the inhibition ofQuorum sensing of Pseudomonas aeruginosa and Chromobacterium violaceumR.E. D?Almeida a,1, R.D.I. Molina a,1, C.M. Viola a,1, M.C. Luciardi a,b, C. Nieto Peñalver b,c, A. Bardón b,d, M.E. Arena a,b,*a INBIOFAL (Instituto de Biotecnología Farmacéutica y Alimentaria), CONICET, Ayacucho 471, Tucumán 4000, Argentinab Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Ayacucho 471, Tucumán 4000, Argentinac PROIMI ? CONICET, Argentinad INQUINOA ? CONICET, Argentina1 Authors have the same participationAbstractQuorum sensing (QS) is a cell-to-cell signaling communication system that controls the virulence behavior of a broad spectrum of bacterial pathogens, participating also in the development of biofilms, responsible of the antibiotic ineffectiveness in many infections. Therefore, QS system is an attractive target for antimicrobial therapy. In this study, we compare the effect of seven structurally related coumarins against bacterial growth, biofilm formation and elastase activity of Pseudomonas aeruginosa. In addition, the anti-pathogenic capacity of the seven coumarins was evaluated on the wild type and the biosensorstrain of Chromobacterium violaceum.The comparative study of coumarins showed that molecules with hydroxyl groups on the aromatic ring displayed higher activity on the inhibition of biofilm formation of P. aeruginosa over coumarins with substituents in positions 3 and 4 or without the double 3,4-bond. These 3 or 4-hydroxylated positions caused a decrease in the anti-biofilm activity obtained for coumarin. However, the hydroxyl group in position 3 of the pyrone ring was important for the inhibition of C. violaceum QS and elastolytic activity of P. aeruginosa. The effects observed were active independently of any effect on growth. According to our results, coumarin and its hydroxylated derivatives represent an interesting group of compounds to use as antivirulence agents against the human pathogen P. aeruginosa.