Screening of Bacterial Insecticides against Musca domestica L. (Diptera: Muscidae)

MUSTAFA YAMAC; ELIF SAHIN; EMRE CEYHAN; M. JULIA AMOROSO; S. ANTONIO CUOZZO; SEVIL PILATIN

Fresenius Environmental Bulletin

Technische Universitat Munchen

Lugar: Germany; Año: 2009 vol. 19

1018-4619

In this study, total of 396 bacterial isolates were obtained from soil samples of Eskisehir, Turkey and were screened for their antimicrobial and insecticidal activity. Only the isolates displaying weak or no activity, the total of 322, were selected for the insecticidal activity studies. Two of them (39.02 and 42.04) showed insecticidal activity on Musca domestica larvae and on adults with topical application. Insecticidal activity of the isolates was compared to those of standard insecticidal compounds produced by actinomycetes (antimycin A, cycloheximide and nikkomycin Z) and insecticidal compounds producing collection strains (Streptomyces avermitilis NRRL 8165 and S. pactum NRRL 5230). In primary screening program, mortality rates of isolates 39.02 and 42.04 on M. domestica larvae were 95% and 75%, respectively. Insecticidal compounds produced by isolates 39.02 and 42.04 were more effective by topical application than ingestion on adult flies. But, they did not show any insecticidal activity on the pupae and all pupae were hatched into adult flies. The genotoxic potentials of the crude extracts of the active isolates were studied with Umu test using Salmonella typhimurium NM2009 and NM3009 strains. The results showed that all compounds did not have mutagenic activity. 16S rRNA analysis of isolates 39.02 and 42.04 showed that they belong to genera Achromobacter and Streptomyces, respectively.