PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Chromate removal by yeasts isolated from sediments
Autor/es:
VILLEGAS, LILIANA B.; FERNÁNDEZ, PABLO M.; AMOROSO, MARÍA J.; FIGUEROA, LUCÍA I. C.
Revista:
BIOMETALS
Editorial:
Springer Science+Business Media, LLC
Referencias:
Año: 2008 p. 591 - 600
ISSN:
0966-0844
Resumen:
Abstract Twenty-one yeast-like microorganismswere isolated from tannery effluents and from anickel–copper mine in Argentina. They were tested fortheir Cu(II), Ni(II), Cd(II) and Cr(VI) tolerance inqualitative assays on solid medium. Three isolateswere selected for their multiple tolerance to thedifferent heavy metals and highest tolerance to Cr(VI).According to morphological and physiological analy-sis and 26S rDNA D1/D2 domain sequences theisolates were characterized as: Lecythophora sp. NGV-1, Candida sp. NGV-9 and Aureobasidium pullulansVR-8. Resistance of the three strains to high Cr(VI)concentrations and their ability to remove Cr(VI) wereassessed using YNB-glucose medium supplementedwith 0.5 and 1 mM Cr(VI). Chromate removal activitywas estimated by measuring remaining Cr(VI) con-centration in the supernatant using the colorimetric1,5-diphenylcarbazide method and total chromiumwas determined by flame atomic absorption spectros-copy. The results indicate that the initial Cr(VI)concentration negatively influenced growth and thespecific growth rate but stimulated the metabolicactivity of the three strains; resistance to Cr(VI) bythese strains was mainly due to reduction of Cr(VI)rather than chromium bioaccumulation. This studyshowed the potential ability of these strains as tools forbioremediation of Cr(VI) from contaminated sites.