PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis
Autor/es:
TORRES S; BAIGORÍ M; SWATHY S.L.; ASHOK PANDEY; CASTRO G.R
Revista:
FOOD RESEARCH INTERNATIONAL
Editorial:
ELSEVIER
Referencias:
Año: 2008
ISSN:
0963-9969
Resumen:
New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized.
The enzyme named as type II esterase showed an optimal activity in the temperature range 6065 C and
pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 C), but remarkable stable at extremely
alkaline pH, retaining 100% of its activity at pH 10.011.0. Furthermore, the esterase showed high
stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are
inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The
immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl
acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature
(28 C) and with a very low amount of enzyme (4.6 105 mg ml1), indicates a high potential for type II
esterase in isoamyl acetate synthesis for production purposes.Bacillus licheniformis S-86 strain was characterized.
The enzyme named as type II esterase showed an optimal activity in the temperature range 6065 C and
pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 C), but remarkable stable at extremely
alkaline pH, retaining 100% of its activity at pH 10.011.0. Furthermore, the esterase showed high
stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are
inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The
immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl
acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature
(28 C) and with a very low amount of enzyme (4.6 105 mg ml1), indicates a high potential for type II
esterase in isoamyl acetate synthesis for production purposes.p-nitrophenyl
acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature
(28 C) and with a very low amount of enzyme (4.6 105 mg ml1), indicates a high potential for type II
esterase in isoamyl acetate synthesis for production purposes.n-hexane. The resulting ester yield (42.8%), obtained at a low temperature
(28 C) and with a very low amount of enzyme (4.6 105 mg ml1), indicates a high potential for type II
esterase in isoamyl acetate synthesis for production purposes.105 mg ml1), indicates a high potential for type II
esterase in isoamyl acetate synthesis for production purposes.