Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications

COLÍN, VERÓNICA; BAIGORI, MARIO DOMINGO; PERA, LICIA MARÍA

AMB Express

Springer-Verlag

Año: 2013 vol. 3 p. 1 - 13

2191-0855

Current problems of filamentous fungi fermentations and their further successfuldevelopments as microbial cell factories are dependent on control fungal morphology. In thisconnection, this work explored new experimental procedures in order to quantitatively checkthe potential of some culture conditions to induce a determined fungal morphology byaltering both hyphal morphology and conidia adhesion capacity. The capacity ofenvironmental conditions to modify hyphal morphology was evaluated by examining theinfluence of some culture conditions on the cell wall lytic potential of Aspergillus niger MYA135. The relative value of the cell wall lytic potential was determined by measuring a cellwall lytic enzyme activity such as the mycelium-bound â-N-acetyl-D-glucosaminidase (Mb-NAGase). On the other hand, the quantitative value of conidia adhesion was considered as anindex of its aggregation capacity. Concerning microscopic morphology, a highly negativecorrelation between the hyphal growth unit length (lHGU) and the specific Mb-NAGaseactivity was found (r=−0.915, P