PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
Autor/es:
SANCHEZ L.A.; GONZALEZ-SIERRA, M.; SIÑERIZ F.; DELGADO O.D.
Revista:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 p. 1 - 9
ISSN:
0959-3993
Resumen:
Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperatures (8°C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from Ser. proteamaculans culture was only detected at 25°C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. MIC values determined by microdilution experiments varied in the range between 0.01 - 0.78 µg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H / 13C NMR approaches. Ultra-structure effects on sensitive E. coli 35218 were observed by transmission electron microscopy at different inhibition stages. Ser. proteamaculans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing an interesting biotechnology potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria. The work also highlights bioreactor production and purification procedure as well as cold environments as a suitable source of microorganisms with ability to produce cold-active biomolecules of biotechnological interest