PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Unraveling the decolourizing ability of yeast isolates from dye-polluted and virgin environments: an ecological and taxonomical overview
Autor/es:
HIPÓLITO F. PAJOT; OSVALDO D. DELGADO; LUCÍA I.C. DE FIGUEROA; JULIA I. FARIÑA
Revista:
ANTON LEEUW INT. J. G.
Editorial:
SPRINGER
Referencias:
Lugar: Dordrecht; Año: 2011 vol. 99 p. 443 - 456
ISSN:
0003-6072
Resumen:
Microcosm assays with dye-amendedculture media under a shot-feeding strategy allowedus to obtain 100 yeast isolates from the wastewateroutfall channel of a dyeing textile factory in Tucuma´n(Argentina). Meanwhile, 63 yeast isolates wereobtained from Phoebe porphyria (Laurel del monte)samples collected from Las Yungas rainforest (Tucuma´n), via a classical isolation scheme. Isolatedyeasts, both from dye-polluted and virgin environments,were compared for their textile dye decolourizationability when cultured on solid and liquidmedia. Nine isolates from wastewater and 17 fromLas Yungas showed the highest decolourizationpotential on agar plates containing six differentreactive dyes, either alone or as a mixture. Fiveyeasts from each environment were further selectedon the basis of their high dye removal rate inVilmafix Red 7B-HE- or Vilmafix Blue RR-BBamendedliquid cultures. Yeasts from wastewatershowed slightly higher decolourization percentagesafter 36 h of culture than yeasts from Las Yungas(98–100% vs. 91–95%, respectively). However, isolatesfrom Las Yungas exhibited higher specificdecolourization rates than isolates from effluents(1.8–3.0 vs. 0.9–1.3 mg g-1h-1, respectively). Allselected isolates were first grouped according tomicrosatellite-PCR analysis and representative isolatesfrom each group were subsequently identifiedbased on the 26S rRNA gene sequence analysis.Yeasts from wastewater were identified as theascomycetous Pichia kudriavzevii (100%) and closelyrelated to Candida sorbophila (99.8%), whilstyeasts from Las Yungas were identified as thebasidiomycetous Trichosporon akiyoshidainum andTrichosporon multisporum. It is suggested that findingsconcerning yeast selection during screeningprograms for dye-decolourizing yeasts may beexplained in the light of the copiotroph-oligotrophmicroorganisms rationale.