PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Poly(B-hydroxybutyrate) accumulation by a Halomonas sp. Isolated from Bolivian Saline Deserts.
Autor/es:
QUILLAGUAMAN J; DELGADO O; MATTIASSON B; HATTI-KAUL R
Revista:
ENZYME AND MICROBIAL TECHNOLOGY
Editorial:
Elsevier
Referencias:
Lugar: Londres; Año: 2006 vol. 38 p. 148 - 154
ISSN:
0141-0229
Resumen:
The moderate halophile Halomonas boliviensis , isolated from a Bolivian saline soil sample, was able to accumulate poly(_-hydroxybutyrate) (PHB) when grown under conditions of nutrient limitation and excess carbon source. The concentration of sodium chloride in the medium influenced the cell-growth, -size, and rate of PHB accumulation. Cultivation in shake flasks led to a PHB accumulation of about 54 wt % with respect to cell dry weight at 4.5% (w/v) NaCl in a medium with butyric acid and sodium acetate as carbon sources. The production of PHB was substantially improved to a maximum value of 88 wt.% during cultivation under controlled conditions of pH and oxygen concentration in a fermentor. The use of glucose and sucrose, respectively, as carbon source could also lead to the production of PHB at an average level of 55 wt.%.