PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Chromate reductase activity in Streptomyces sp. MC1
Autor/es:
POLTI M.A.; AMOROSO, M. J.; ABATE, C. M.
Revista:
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Editorial:
MICROBIOL RES FOUNDATION
Referencias:
Año: 2010 p. 11 - 18
ISSN:
0022-1260
Resumen:
Biological transformation of Cr(VI) to Cr(III) by enzymatic reduction may provide a less costly and more environmentally friendly approach to remediation. In a previous report a Cr(VI) resis-tant actinomycete strain, Streptomyces sp. MC1, was able to reduce Cr(VI) present in a syn-thetic medium, soil extract and soil samples. This is the fi  rst time optimal conditions such as pH, temperature, growth phase and electron donor have been elucidated in vitro for Cr(VI) reduction by a streptomycete. Chromate reductase of  Streptomyces sp. MC1 is a constitutive enzyme which was mainly associated with biomass and required NAD(P)H as an electron donor. It was active over a broad temperature (19Ȃ39›C) and pH (5Ȃ8) range, and optimum conditions were 30›C and pH 7. The enzyme was present in supernatant, pellet and cell free extract. Bioremedia-tion with the enzyme was observed in non-compatible cell reproduction systems, conditions frequently found in contaminated environments.