Differential display of Ilex paraguariensis leaf mRNAs regulated by plant water stress

ACEVEDO, RAÚL M; DISTEL, MAURO; STEIN, JULIANA; ESPINOZA, FRANCISCO; PESSINO, SILVINA; SANSBERRO, PEDRO A.

Montreal

Congreso; Plant Biology 2010; 2010

American Society of Plant Biologists - Canadian Society of Plant Physiologists

Ilex paraguariensis plants are often exposed to water stress during the summer season, which leads to leaf abscission and yield reduction. To identify drought-responsive genes, transcriptomes were studied in plants subjected to different øsoil (-0.1, -1, -2, -3 MPa, re-watering at -3 MPa). Approximately 80.000 transcript-derived fragments (TDFs) were screened. Fourteen TDFs with differential expression between non-stressed and stressed plants were identified, isolated and cloned. Transcriptome profiling showed that twelve TDFs were induced, while two were repressed during the stress period. Induced TDFs sequences showed significant homology with genes encoding ATP-binding cassette transporter (At5g44316), succinate dehydrogenase (NP001146352.1), glycine-rich cell wall structural protein (XM002528173.1), DC1-domain containing protein (XP002323288.1), replication protein A2(XP002268721.1),seed imbibition protein 1 (AC157350.29), ubiquitin conjugated enzyme family member (XM002330687.1), conserved hypothetical proteins (XP002521269.1, XM002520720.1), and predicted hypothetical proteins (XM002272199.1, XM002279385.1). Repressed TDFs sequences showed significant homology with genes encoding protein phosphatase 2C PPH1 (NM179128.1) and Ty1-copia-like retrotransposon (ORSiTERT00100078). A 810 F1 pseudo-test cross mapping population was established by crossing SI-67 x SI-49, non-tolerant and tolerant genotypes, respectively. The construction of an AFLP-based genetic map is now under development, which will be used to identify water-stress related QTLs and locate the activated-repressed sequences in the I. paraguariensis genome.