INFERENCES OF GENETIC ANDEPIGENETIC CHANGES OCCURRED DURING THE HYBRIDIZATION AND POLYPLOIDIZATION THAT ORIGINETD Arachis Hypogaea

VIVIANA, SOLÍS NEFFA; PAREDES, ESTEBAN N; GERMÁN ROBLEDO; ALEJANDRA, GARCÍA

Congreso; XXII International Congress of Genetics (ICG); 2018

Polyploidy and hybridization are the most common evolutionary processes in higher plants withimmediate effects on genome structure. These processes would produce a considerable amountof genetic shock in the nucleous, leading to genetic and epigenetic modifications. We comparedthe AFLP and MS-AFLP profiles of the two subspecies of the allotetraploid Arachis hypogaea(AABB) with those of the wild allotetraploid A. monticola (AABB), two synthetic diploid hybrids(AB), and the diploid parental species A. ipaënsis (BB) and A. duranensis (AA), aiming to analyzethe genomic and epigenetic changes occurred in A. hypogaea due to hybridization, polyploidyand domestication/diversification processes. The results of the comparisons between diploids vs.hybrids, hybrids vs. A. monticola, and A. monticola vs. both subspecies of A. hypogaea revealedsimilar frequency of loci gain and loss. All these changes affected both genomes, but A genomesuffered more loci loss. The comparisons of methylation states of loci between diploids andhybrids revealed that the change frequency in both A and B genomes were similar, but locidemethylation was more frequent in A and methylation in B. The comparisons between hybridsand wild allotetraploid A. monticola, and A. monticola vs both subspecies of A. hypogaea showedsimilar frequency of state change. However, loci demethylation was more frequent inpolyploidyzation and methylation in domestication/diversification processes. This study suggeststhat genomic and epigenetic changes due to hybridization, polyploidyzation anddomestication/diversification in the origin A. hypogaea were similar in magnitude, but affected theA and B genomes differently.