IBONE   05434
INSTITUTO DE BOTANICA DEL NORDESTE
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EXPANSIN PROTEIN INVOLVED IN SOYBEAN GERMINATION AND ITS CONTROL BY ABA
Autor/es:
MONTECHIARINI H. NIDIA; DELGADO LUCIANA; PIERELLA JUAN; PALMIERI, REGINA; FERRERO, A.; ROSETTI, I.; ELIGIO N. MORANDI; GOSPARINI, CARLOS
Lugar:
Rosario
Reunión:
Congreso; 5. XVI Congreso ? XXXIV Reunión Anual. Sociedad Biología de Rosario; 2014
Resumen:
Seed germination begins with water uptake and ends with radicle protrusion through seed coat. This process is driven by the elongation of a definite region from embryo axis known as Elongation Zone (EZ) without cellular division.  This expansion occurs due to changes in cell wall plasticity, controlled metabolically, allowing the irreversible increase of volume. This process was proposed to be mainly controlled by a group of non hydrolytic proteins, known generically as Expansins (EXP). On the other hand, the abscisic acid (ABA) is a known germination inhibitor and its action would operate on initial stages, preventing the relaxation of the cell wall and the subsequent uptake of water. The objectives of this work were to evaluate the expression level of EXP on soybean germination and under ABA treatments. Embryonic axes of soybean (Glycine max L. Merr) at physiological maturity (45 day after anthesis) were incubated in distilled water (DA) or in 50 µM ABA. Specific primers were designed from orthologous genes of Arabidopsis thaliana and Medicago truncatula. cDNA from EZ of germinated axes was used as template in PCR reaction. A fragment of 870 bp was cloned and sequenced showing high homology with EXP1 of soybean. The mRNA expression levels were evaluated by qRT-PCR from ZE of embryonic axes incubated at 0, 6 and 12 h in DA or ABA, using as housekeeping gene Glyma05g37470 used previously by ?.etal. Expression studies indicated that EXP1 transciption is triggered during germination produced over DA incubation, but decreases under ABA treatment. These results suggest a role of EXP1 on germination process probably on cell wall relaxation, acting downstream of ABA signal.