Genetic transformation of Lotus tenuis with NHX1 antiporter gene

ESPASANDIN FABIANA DANIELA; ALVAREZ MAYRA YANET; SANSBERRO PEDRO ALFONSO; MARIANA DEL VAS; DÍAZ PALEO ANTONIO; AFFINITO AGOSTINA; FRANCISCO LUNA

Otro; XXXIII Reunión Argentina de Fisiología Vegetal; 2021

Sociedad Argentina de Fisiologia Vegetal

Salt stress negatively affects plants due to osmotic effects, ion toxicity and nutritional disorders (2). One of the strategies to protect from this stress is to avoid ion toxicity by compartmentalizationof Na+ in vacuoles mediating by Na+/H+ antiporters of the NHX type located in the tonoplast (3). To study the importance of NHX1 antiporter of L. tenuis (LtNHX1) in plant salt tolerance, by meanof Agrobacterium tumefaciens-mediated transformation (1), we obtained L. tenuis plants overexpressing or silencing LtNHX1. The first binary vector contained the LtNHX1 coding region under thecontrol of CaMV35S constitutive promoter (35S:NHX1), while the other contained an inverted repeat of a 272-bp fragment of LtNHX1, to trigger post-transcriptional gene silencing under thecontrol of the CaMV35S promoter (35S:PTGS). Both vectors carry the bialaphos acetyltransferase gene (bar), which confers resistance to glufosinate-ammonium