TISSUE CULTURE OF ACHYROCLINE SPP. (ASTERACEAE)

SANSBERRO, PA; KOTIK, D.; TARRAGÓ, J; LUNA, C.; MROGINSKI, L.A

Protocols on Micropropagation of Medicinal Plants

Jahangirnagar University

Lugar: Bangladesh; Año: 2007;

Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf 14 explants of Achyrocline satureioides and Achyrocline alata. Organogenesis was induced 15 from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 16 30 g¡¤L-1) containing different combinations of 6-benzyladenine (BA) and ¥á- 17 naphtalenacetic acid (NAA) under 116 ¥ìmol¡¤m-2¡¤s-1 photosynthetic photon flux density 18 (PPFD), photoperiod 14 h and at 27¡¾2¨¬C. Organogenesis was markedly influenced by 19 the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitroAchyrocline satureioides and Achyrocline alata. Organogenesis was induced 15 from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 16 30 g¡¤L-1) containing different combinations of 6-benzyladenine (BA) and ¥á- 17 naphtalenacetic acid (NAA) under 116 ¥ìmol¡¤m-2¡¤s-1 photosynthetic photon flux density 18 (PPFD), photoperiod 14 h and at 27¡¾2¨¬C. Organogenesis was markedly influenced by 19 the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro-1) containing different combinations of 6-benzyladenine (BA) and ¥á- 17 naphtalenacetic acid (NAA) under 116 ¥ìmol¡¤m-2¡¤s-1 photosynthetic photon flux density 18 (PPFD), photoperiod 14 h and at 27¡¾2¨¬C. Organogenesis was markedly influenced by 19 the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro¥ìmol¡¤m-2¡¤s-1 photosynthetic photon flux density 18 (PPFD), photoperiod 14 h and at 27¡¾2¨¬C. Organogenesis was markedly influenced by 19 the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro¡¾2¨¬C. Organogenesis was markedly influenced by 19 the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitroA. alata. After 30 d of culture in MS plus NAA at 0.5 ¥ìM and 20 BA at 5 ¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro¥ìM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 21 12¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro¡¾12, 50¡¾14.4, and 94¡¾2.8 %, respectively. In A. satureioides the regeneration was 22 similar for every tested explant and varied between 64 and 83 %. The number of buds 23 formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In 24 order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitroin vitro rooting, regenerative leaves were sub cultured from the best 25 induction medium in MS lacking plant growth regulators for the same periods. Every 26 plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitroin vitro was phenotypically normal and successfully hardened to ex vitro 27 conditions. An experimental field plot with 60-day-old in vitro regenerated plants was 28 established.in vitro regenerated plants was 28 established.