Cryopreservation of Ilex immature zygotic embryos

MROGINSKI, L. A.; DOLCE, N.; SANSBERRO, P.A.; LUNA, C.; GONZALEZ, A.M.; REY, H.Y.

Plant Embryo Culture, Methods and Protocols

Humana Press - Springer

Lugar: New York; Año: 2011; p. 215 - 225

Tropical Ilex species have recalcitrant seeds. This work describes protocols demonstrating the feasibility of long-term conservation Ilex brasiliensis, I. brevicuspis, I. dumosa, I. microdonta, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos  were aseptically removed from the seeds and precultured (7 days) in the dark, at 27¡¾