IBONE   05434
INSTITUTO DE BOTANICA DEL NORDESTE
Unidad Ejecutora - UE
artículos
Título:
Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants
Autor/es:
VILA, SK; GONZALEZ, AM; REY ,HY; MROGINSKI LA
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: 34(1):7-13 ; Año: 2010 vol. 34 p. 7 - 13
ISSN:
0327-9545
Resumen:
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic
embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 ìM thidiazuron or 0.45
ìM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized
in four morphological classes based on the presence of single or fused embryos and if they remained
united or not to the original explant; that were evaluated histologically. The somatic embryos of every category
were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically
normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually
their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos
subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conversion
rate in the others classes was associated to poorly developed shoot and root meristematic areas or with
their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate