Bacterial Contamination in Ilex dumosa (Aquifoliaceae) Cultures: Antibiotic Treatment.

LUNA, C.; COLLAVINO, M.; SANSBERRO, P.A.; MROGINSKI L.

ACTA HORTICULTURAE

The Netherlands

Año: 2009 p. 97 - 102

0567-7572

A range of commercially antibiotics were tested on nodal segments-culture of Ilex dumosa for their ability to control bacterial (Gram negative) contamination. Thirteen treatment with 4 antibiotic were tested: streptomycin (200 mg.ml-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg·ml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTIONfor their ability to control bacterial (Gram negative) contamination. Thirteen treatment with 4 antibiotic were tested: streptomycin (200 mg.ml-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg·ml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg·ml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1), cefotaxime (0.25, 0.50 and 1 mg·ml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTIONwere cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 ìM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 ìmol·m-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-2·s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. INTRODUCTION