IBONE   05434
INSTITUTO DE BOTANICA DEL NORDESTE
Unidad Ejecutora - UE
artículos
Título:
A reference floral transcriptome of sexual and apomictic Paspalum notatum
Autor/es:
ORTIZ JPA; PODIO M; LEBLANC O; SIENA LA; STEIN J; REVALE, S; DELGADO L; PESSINO SC
Revista:
BMC GENOMICS
Editorial:
BIOMED CENTRAL LTD
Referencias:
Lugar: Londres; Año: 2017
ISSN:
1471-2164
Resumen:
Background: Paspalum notatum Flügge is a subtropical grass native to South America, which includes sexualdiploid and apomictic polyploid biotypes. In the past decade, a number of apomixis-associated genes werediscovered in this species through genetic mapping and differential expression surveys. However, the scarceinformation on Paspalum sequences available in public databanks limited annotations and functionalpredictions for these candidates.Results: We used a long-read 454/Roche FLX+ sequencing strategy to produce robust reference transcriptome datasetsfrom florets of sexual and apomictic Paspalum notatum genotypes and delivered a list of transcripts showing differentialrepresentation in both reproductive types. Raw data originated from floral samples collected from premeiosis to anthesiswas assembled in three libraries: i) sexual (SEX), ii) apomictic (APO) and iii) global (SEX + APO). A group of physicallysupportedPaspalum mRNA and EST sequences matched with high level of confidence to both sexual and apomicticlibraries. A preliminary trial allowed discovery of the whole set of putative alleles/paralogs corresponding to 23 previouslyidentified apomixis-associated candidate genes. Moreover, a list of 3,732 transcripts and several co-expression andprotein ?protein interaction networks associated with apomixis were identified.Conclusions: The use of the 454/Roche FLX+ transcriptome database will allow the detailed characterization of floralalleles/paralogs of apomixis candidate genes identified in prior and future work. Moreover, it was used to revealadditional candidate genes differentially represented in apomictic and sexual flowers. Gene ontology (GO) analyses ofthis set of transcripts indicated that the main molecular pathways altered in the apomictic genotype correspond tospecific biological processes, like biotic and abiotic stress responses, growth, development, cell death and senescence.This data collection will be of interest to the plant reproduction research community and, particularly, to Paspalumbreeding projects.