IBONE   05434
INSTITUTO DE BOTANICA DEL NORDESTE
Unidad Ejecutora - UE
artículos
Título:
Cryopreservation of Arachis pintoi (Leguminosae) seeds
Autor/es:
REY ,HY; MROGINSKI ,LA
Revista:
SEED SCIENCE AND TECHNOLOGY
Editorial:
International Seed Testing Association - ISTA
Referencias:
Lugar: Virum, Denmark; Año: 2008
ISSN:
0251-0952
Resumen:
Summary. Studies on the storage of seeds of the diploid cytotype (2n=2x= 20 chromosomes) of Arachis pintoi (Leguminosae) were conducted to preserve germplasm for the long term in liquid nitrogen (-196°C). The best cryo-procedure  comprised: desiccation of the seeds to 4% water content (14 days at 40°C) and, subsequently, direct cooling in nitrogen liquid. The cryotubes were then rapidly warmed in a water bath at 38°C. With this treatment, after 7 days in a germinator up to 90% of the seeds germinated.