Bacterial Contamination in Ilex dumosa (Aquifoliaceae) Cultures: Antibiotic Treatment

LUNA, C.; COLLAVINO, M.; SANSBERRO, PA; MROGINSKI, L.A

ACTA HORTICULTURAE

Año: 2007

0567-7572

A range of commercially antibiotics were tested on nodal segments-culture of Ilex dumosa for their ability to control bacterial (Gram negative) contamination. 13 treatment with 4 antibiotic were tested: Streptomycin(200 mg·ml-1), Chloramphenicol (50 mg·ml-1), Cefotaxime (0.25, 0.50 and 1 mg·ml-1) and combinations between Ampicilline (200 mg·ml-1) + Chloramphenicol (50 mg·ml-1), Streptomycin(200 mg·ml-1) + Cefotaxime (0.50 mg·ml-1) , Cefotaxime (0.25, 0.50 and 1 mg·ml -1) + Ampicilline (200 mg·ml-1) or Chloramphenicol (50 mg·ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4 MS), with 1,5% sucrose and 20μM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 hr ) under 27 ± 2 ºC with lighting (cool white fluorescent light) at 116 μmol·m-2·s-1 and 14 hr photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg·ml-1Cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots.