Desinfección de cariopses y regeneración de plantas de Spartina argentinensis

BUENO MS; FELDMAN SR; ORTIZ JPA

Ciencia e Investigación Agraria

Pontificia Universidad Católica de Chile

Lugar: Santiago de Chile; Año: 2007 vol. 34 p. 231 - 236

0718-1620

A protocol for disinfection and in vitro regeneration of Spartina argentinensis was developed. The lowest percentage of contamination was obtained after treating caryopses with 90° ethanol for 60 s and 20 min immersion in 2.5% sodium hypochlorite (NaOCl) plus 0.1% Tween 20. Murashige and Skoog (MS) media was used for callus induction, supplemented with 0.5 mg·L -1 of 2,4 dichlorophenoxy acetic (2,4-D) and 1 mg·L -1 of indole-3-acetic acid (IAA). The highest proportion (20%) of shoot regeneration was achieved with MS media plus 0.25 mg·L -1 of bencyl aminopurine (BAP), whereas 0.5 mg·L -1 naphtalenacetic acid (NAA) was used for root induction. Plants were regenerated via organogenesis. To our knowledge, this is the fi rst report of a protocol for in vitro plant regeneration of S. argentinensis, and it is the initial stage required for obtaining somaclonal variations.