IBONE   05434
INSTITUTO DE BOTANICA DEL NORDESTE
Unidad Ejecutora - UE
artículos
Título:
Genetic response of Paspalum plicatulum to genome duplication
Autor/es:
WEIHMÜLLER E; BELTRÁN C; SARTOR ME; ESPINOZA F; SPAMPINATO C; PESSINO SC
Revista:
GENETICA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2014 vol. 142 p. 227 - 234
ISSN:
0016-6707
Resumen:
Abstract Paspalum plicatulum is a perennial rhizomatous
grass with natural diploid and polyploid cytotypes. In
this study, we investigated the occurrence of sequence
polymorphisms arising immediately after genome autoduplication
in this species. Two mixoploid plants (4C and 7D)
were previously obtained through colchicine treatment of
seeds generated by open pollination of a diploid plant
(H14-2x). Diploid and tetraploid sectors from both mixoploids
were dissected to generate two ploidy series (4C-
2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were
generated from the maternal plant H14-2x, both ploidy
series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid
plant (C1) produced by selfing 7D-4x. Our results indicate
that immediately after polyploidization P. plicatulum suffers
genetic rearrangements affecting *28?38 % of the
genome. Band gain and loss were equally prevalent at a
statistically significant level. At least 5.62 % of the genome
experimented recurrent genetic variation in a non-random
basis with a confidence of 94.88 %. A significant
proportion of novel bands (36 out of 195; 18.4 %) was
detected in the C1 tetraploid plant. Half of these bands
were not amplified in either H14-2x or 7D-4x, while the
remainders were present in H14-2x but absent in 7D-4x.
Our results indicate the occurrence of a considerable
number of genetic changes in P. plicatulum immediately
after polyploidization, some of which were recurrently
detected in different independent events. Moreover, we
confirmed that after polyploidization, lost ancestral alleles
were spontaneously recovered in further generations, a
phenomenon previously reported by other research groups.