Putrescine accumulation in Arabidopsis thaliana transgenic lines enhances tolerance to dehydration and freezing stress

ANALÍA I. ALET, DIEGO H. SANCHEZ, JUAN C. CUEVAS, SECUNDINO DEL VALLE, TERESA ALTABELLA, ANTONIO F. TIBURCIO, FRANCISCO MARCO, ALEJANDRO FERRANDO, FABIANA D. ESPASANDÍN, MARÍA E. GONZÁLEZ, PEDRO CARRASCO, OSCAR A. RUIZ

Plant Signalling and Behavior

Landes biosciences

Lugar: Bonn; Año: 2010 vol. 6 p. 181 - 189

1559-2324

Polyamines have been globally associated to plant responses to abiotic stress. Particularly, putrescine has been related to a better response to cold and dehydration stresses. It is known that this polyamine is involved in cold tolerance, since Arabidopsis thaliana plants mutated in the key enzyme responsible for putrescine synthesis (arginine decarboxilase, ADC; EC 4.1.1.19) are more sensitive than the wild type to this stress. Although it is speculated that the overexpression of ADC genes may confer tolerance, this is hampered by pleiotropic effects arising from the constitutive expression of enzymes from the polyamine metabolism. Here, we present our work using A. thaliana transgenic plants harboring the ADC gene from oat under the control of a stress-inducible promoter (pRD29A) instead of a constitutive promoter. The transgenic lines presented in this work were more resistant to both cold and dehydration stresses, associated with a concomitant increment in endogenous putrescine levels under stress. Furthermore, the increment in putrescine upon cold treatment correlates with the induction of known stress-responsive genes, and suggests that putrescine may be directly or indirectly involved in ABA metabolism and gene expression.