INTEMA   05428
INSTITUTO DE INVESTIGACIONES EN CIENCIA Y TECNOLOGIA DE MATERIALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ELECTROCHEMISTRY OF EXTRACELULAR CYTOCHROME OMC FROM THE ELECTRO-ACTIVE BACTERIA Geobacter sulfurreducens
Autor/es:
MV ORDÓÑEZ; SCHROTT GD; INFOSSI P; LOJOU E; BUSALMEN JP
Lugar:
Mar del Plata
Reunión:
Congreso; - SAIB - 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015
Institución organizadora:
SAIB (Sociedad Argentina de Investigación en Bioquímica y Biología Molecular)
Resumen:
Geobacter sulfurreducens isan anaerobic gram (?) bacteria capable of using extracellular insolubleelectron acceptor like Fe (III) (hydr)oxides during respiration as well as apolarized electrode thus producing an electric current. Elucidating themolecular mechanisms behind these modes of respiration is significant for thedevelopment of clean energy technologies. Soluble redox proteins like externalcytochromes play a key role in the electron transport and reduction ofinsoluble electron acceptor in Geobacter. In this work we study externalsoluble cytochromes type c (Omc or Outter membrane cytochromes) of theextracellular matrix of G. sulfurreducens electro-active biofilms developedover polarized electrodes. For this we performed the purification andcharacterization of small cytochrome Omc12 involved in the current productionmechanism. Our results showed that Omc12 is a small protein of 12 kDa with aredox behavior typical for mono-heme cytochromes type C and an electrochemical midpointpotential of -135mV. Its interaction with the electrode surface as well as itsredox activity changed when electrode surface or physic-chemical conditionswere modified. Characterization of this molecule allows us to better understandthe Direct Electron Transfer mechanism in this bacteria and its possibleapplication in biosensing as well as microbial fuel cell technologies.